txnrd1 rabbit monoclonal antibody  (Boster Bio)

Journal: Advanced Science

Article Title: Glabridin‐Gold(I) Complex as a Novel Immunomodulatory Agent Targeting TrxR and MAPK Pathways for Synergistic Enhancement of Antitumor Immunity

doi: 10.1002/advs.202504729

Figure Lengend Snippet: Scheme 1. The glabridin-gold(I) complex 6d enhances tumor immuno- genicity by targeting TrxR while simultaneously remodeling the immuno- suppressive microenvironment through MAPK pathway inhibition (Image created with BioRender; M2 is M2-type macrophage in this image).

Article Snippet: Anti-TXNRD1 Rabbit monoclonal Antibody was purchased from Boster Biological Technology (USA). p-MEK1/2 antibody was purchased from Santa Cruz Biotechnology, Inc (USA).

Techniques: Inhibition

Journal: Advanced Science

Article Title: Glabridin‐Gold(I) Complex as a Novel Immunomodulatory Agent Targeting TrxR and MAPK Pathways for Synergistic Enhancement of Antitumor Immunity

doi: 10.1002/advs.202504729

Figure Lengend Snippet: Scheme 2. Construction of the glabridin-gold(I) complex 6d, designed to target both MAPK and TrxR.

Article Snippet: Anti-TXNRD1 Rabbit monoclonal Antibody was purchased from Boster Biological Technology (USA). p-MEK1/2 antibody was purchased from Santa Cruz Biotechnology, Inc (USA).

Techniques:

Journal: Advanced Science

Article Title: Glabridin‐Gold(I) Complex as a Novel Immunomodulatory Agent Targeting TrxR and MAPK Pathways for Synergistic Enhancement of Antitumor Immunity

doi: 10.1002/advs.202504729

Figure Lengend Snippet: Figure 2. TrxR inhibitory activity and ICD effects of complex 6d. A) Fluorescence analysis of TrxR activity in HepG2 cells after 24 h of 6d treatment using a TrxR probe (scale bar = 200 μm). B) Intracellular TrxR activity assay in HepG2 cells after incubation with 6d, 24 h measured using the TrxR Activity Assay Kit. C) Inhibition of purified TrxR by 6d. D) Schematic workflow of the DC maturation assay (Image created with BioRender). E) Flow cytometry analysis of DC maturation following 6d treatment for 24 h. F) Western blot analysis of CRT and HMGB1 expression after 24 h of 6d treatment. G) Time-dependent measurement of ATP release from HepG2 cells after 6d treatment using the ATP Assay Kit. Data are presented as mean ± SD (n = 3); Student’s t-test, compared with DMF group; ns > 0.05, *p < 0.05, **p < 0.01, and ***p < 0.001.

Article Snippet: Anti-TXNRD1 Rabbit monoclonal Antibody was purchased from Boster Biological Technology (USA). p-MEK1/2 antibody was purchased from Santa Cruz Biotechnology, Inc (USA).

Techniques: Activity Assay, Fluorescence, Incubation, Inhibition, Flow Cytometry, Western Blot, Expressing, ATP Assay